Journal: bioRxiv
Article Title: Oligodendrocyte precursor cells exacerbate acute CNS inflammation via macrophage and T cell activation in a mouse model of multiple sclerosis
doi: 10.1101/2024.05.28.596190
Figure Lengend Snippet: A . Time course of OPC depletion in naïve mice. B, C . Fluorescent immunostaining for PDGFRα in the lumbar spinal cord at day 9 ( B ) and quantification of the number of positive cells in 0.08 mm 2 ( C ). Scale bar, 100 µm. n = 9–11 mice. D-G . Fluorescent immunostaining for CC-1, Iba1 and GFAP in the lumbar spinal cord at day 9. Scale bar, 100 µm. Quantification of the number of CC-1 positive cells ( E ) in 0.08 mm 2 .area, relative fluorescence intensity of Iba1 ( F ) and GFAP ( G ) in the white matter area. n = 6–8 mice. H . The numbers of white blood cells (WBCs), red blood cells (RBCs), and the percentages of lymphocytes, monocytes, and neutrophils in the WBCs at day 9. n = 4–5 mice. *** P < 0.001. Data are expressed as mean ± SEM.
Article Snippet: Sections were incubated with primary antibodies at 4 °C overnight (14-20 h) at the following dilutions: anti-PDGFRα (AF1062-SP, goat IgG, 1:250; R&D Systems), anti-APC (Ab-7) Mouse mAb (CC-1) (OP80, mouse IgG, 1:200; Calbiochem®), anti-ionized calcium binding adaptor molecule 1 (Iba1) (019-19741, rabbit IgG, 1:200; Wako Pure Chemical Industries), anti-glial fibrillary acidic protein (GFAP) (ab7260, rabbit IgG, 1:200; Abcam), anti-CD31 (553370, rat IgG, 1:200; BD Biosciences), anti-CD3 (555273, rat IgG, 1:200; BD Biosciences), anti-IL17 (NBP1-76337, rabbit IgG, 1:150; Novus Biologicals), anti-MHC class II (556999, rat IgG, 1:50; BD Biosciences), anti-collagen I (ab21286, rabbit IgG, 1:250; Abcam), anti-CD45 (AF114-SP, goat IgG, 1:50; R&D Systems), anti-CD11c (14-0114-82, Armenian hamster IgG, 1:50; eBioscience).
Techniques: Immunostaining, Fluorescence
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